WP2 overview

Mission

WP2 is a work package to data production in FANTOM5. We aim to produce high quality data with

• Library construction
• Sequencing with NGS
• Data processing
• And those quality control.

With collaboration with other WPs,

• Receive samples from WP1.
  • record sample annotation.
• Technical transfer from WP3.
  • We have been transferred following protocols. -> Protocols
  • Those technical transfers are under controlling with ISO9001 (Quality Management System).
• Transfer data to WP4.
  • Check data quality with WP4.

Overview of WP2 Data Production

Members

• Jun Kawai (Leader)
• Shiro Fukuda (Secretariat) - User:sfukuda
• Thierry Sengstag (IT Coordination)
• Riichiro Manabe (Production management, Flow construction and operation)
• Naoko Suzuki (Production management)
• Sachiko Kato (RNA bank)

Data Prodcution; GeNAS members

• Shunji Yamamori (DDP)
• Hiroo Inaba (QAU)
• Yumiko Yamamoto (PLA)
• Fumi Hori (PCO)
• Tsugumi Kawashima (ANA1)
• Sayaka Sato (ANA1)
• Mizuho Sakai (ANA2)
• Shouhei Noma (ANA3)

Advisor from another WP

• Masayoshi Itho (from WP3)
• Hideya Kawaji (from WP4) - User:Kawaji

Reference

• Our facility of NGS (Next Generation Sequencer)
  • 454 GS FLX Titanium: Roche (x3 machines)
  • 454 GSJunior: Roche (x0 machine -> x1 machine is planned)
  • Genome Analyzer IIx (GAIIx): Illumina (x5 machines -> x2 machines from April 2011.)
  • HiSeq2000: Illumina (x0 machines-> x4 machines from April 2011.)
  • SOLiD 3plus: Life Technologies (x2 machines -> Planned to upgrade to SOLiD4)
  • HeliScope: Helicos (x4 machines (x2 machines working))

• Overview for NGS

Contact

fantom5-wp2@gsc.riken.jp