Extend rat gene models with CAGEscan: Difference between revisions
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(Potential satellite paper to enhance and extend rat gene models using CAGEscan and Helicos CAGE.) |
(Rat CAGEscan libraries made from the same RNA as CNhs10614.) |
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* Some rat gene models miss the real 5′ ends. |
* Some rat gene models miss the real 5′ ends. |
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* [[:Category:Methods_and_Protocols|HelicosCAGE]] and [[CAGEscan]] libraries are available from a “universal” rat RNA preparation. |
* [[:Category:Methods_and_Protocols|HelicosCAGE]] and [[CAGEscan]] libraries are available from a “universal” rat RNA preparation. |
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= Data = |
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== Made from RNA 10009-101B8 == |
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10009-101B8 is the same RNA as used for CNhs10614, the ‘Universal RNA - Rat Normal Tissues’ hCAGE library. |
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* NCig10012: 2 × 54 bp CAGEscan library, 6,903,269 reads. |
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* NCig10071: 2 × 36 bp experimental CAGEscan, 2,893,6176 reads, perhaps better not use it ? |
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* NChi10001: 2 × 51 bp CAGEscan library (HiSeq test run), 9,662,576 reads. |
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= Goal = |
= Goal = |
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Revision as of 16:42, 23 March 2011
Background
- Some rat gene models miss the real 5′ ends.
- HelicosCAGE and CAGEscan libraries are available from a “universal” rat RNA preparation.
Data
Made from RNA 10009-101B8
10009-101B8 is the same RNA as used for CNhs10614, the ‘Universal RNA - Rat Normal Tissues’ hCAGE library.
- NCig10012: 2 × 54 bp CAGEscan library, 6,903,269 reads.
- NCig10071: 2 × 36 bp experimental CAGEscan, 2,893,6176 reads, perhaps better not use it ?
- NChi10001: 2 × 51 bp CAGEscan library (HiSeq test run), 9,662,576 reads.
Goal
Contribute experimental evidence that extends and update the gene models in rat.
