Working Group 2: Blood
BLOOD GROUP
Speakers on Monday 21st February / Tuesday 22nd February David Hume – monocyte/macrophages, several species Daisuke Sugiyama – haemopoietic stem cells, AGM, fetal liver, bone marrow Tomokatsu Ikawa – T cell differentiation Louise Winteringham – erythroid differentiation
SUB-GROUP MEETING ON THURSDAY 24th FEBRUARY 2011
Participants / involvement / interests
Magda Babina - human primary cells, terminal differentiation, plasticity Louise Winteringham - human, mouse erythroid cell lines, differentiation, commitment Michael Rehli - monocytes/macrophages, T cells, human primary cells, terminal differentiation Andreas Lennartsson - granulocytes, terminal differentiation Guojun Sheng - chicken erythroid cells, stem cells, comparative biology Tom Freeman - Microarray netnwork modelling Gabriel Altschuler - lineage definition, function definitions, normal and leukemic cells Win Hide - pathway finger printing Claudio Schneider/Roberto Verardo - human mesenchymal stem, progenitor cells Terry Meehan - cellular ontology Oliver Hofman - cells of unknown origin, functions, ontology John Quackenbush / Jessica Mar - Networks, cell fate transitins, stem and IPS cells, time courses Morana Vitezic – OSC CAGE analysis Silvia Zucchelli – neurobiology, haemopoeitic links especially erythriod genes Juha Kere – mature human T cell subsets, B cells
Outcome of Blood Sub-group meeting • Develop lineage tree of samples (Terry) • Other datasets (Tom) • Previous knowledge (Win) • Blood Wiki page (Al ? → Peter) • Co-ordination (Peter)
Follow-up meetings / discussions (Friday 25th) Possible satellite papers • Haemopoietic stem cells • Haemopoietic vs mesenchumal stem / progenitor cells • Granulopoiesis • Monocytes / macrophages • B / T cells • Erythropoiesis
Status of samples Examination of blood samples collected revealed a range of different cell types from different species. The biggest collection is of human samples. Peter, Louise and Terry attempted to place these samples within the traditional lineage maturation tree. In summary, human samples exist for the following haemopoietic cells: • Haemopoietic stem (CD133+) / early progenitor cells (CD34+) • Common myeloid progenitor cells (CMP) • Granulocyte macrophage progenitors (GMP) • Promyelocytes / myelocytes • Monocytes (CD14+) • Macrophages • Granulocytes (CD15+) • Eosinophils • Basophils • Neutrophils • Natural Killer cells • B cells (CD19+) • T cells (CD4+), T cells (CD8+) • Dendritic cells • Peripheral blood mononuclear cells • Whole Blood
Other related samples • Mouse haemopoietic cells – haemopoietic stem cells (c-kit+, Sca1-, lin-), CMP, GMP • Mesenchumal stem cell samples exist for human, dog, mouse, rat, chicken • Endothelial progenitor cells • Osteoblasts
Points for consideration • There appears to be a good collection of early stem and progenitor cells, especially in the myeloid pathway (HSC, GMP, CMP, promyelocytes / myelocytes).
• There also seems to be a good range of mature myeloid and lymphoid cells (monocytes / macrophages, neutrophils, basophils, eosinophils, NK cells, B cells, T cells).
• However, there are no immature or maturing erythroid cells or megakaryocytes.
• There appear to be gaps in the immature lymphoid compartment.
• Assignment of cells to specific lineages based on single surface markers, or no surface markers, is quite broad (eg CD14+ monocytes, CD15+ granulocytes, CD19+ B cells, CD4+ T cells, CD8+ T cells).
• Other haemopoietic samples may be present within the time courses which could add to the current collection.
• The CAGE data can be compared with other very well curated microarray datasets, eg Haematlas, Immunological Genome, and numerous publications on individual lineages.
