Rinderpest

Rinderpest infection series

Time course ID: human_rinderpest
Sample provider:  Chieko Kai, Hiroki Sato, Misako Yoneda

Introduction

Morbilliviruses including measles virus and rinderpest virus (RPV) are one of the most important pathogens in their respective hosts. In particular, transient strong immunosuppression is the most characteristic feature. Morbillivirus infection induces cell-type-dependent immune responses. However, the molecular mechanisms have not been elucidated. Morbillivirus possesses two nonstructural accessory proteins, V and C. These proteins have been shown to inhibit interferon induction and signaling, although their full functions are unclear. To resolve the complexity and multidimensionality of virus–host interactions, we established recombinant RPV that lacked V and C proteins, and searched for the cell-type-specific transcriptional regulatory network after infection with the FANTOM5 time course analysis. Our study provides a new insight that an accessory protein is one of the virulence factors that define a cell-type-specific response of morbillivirus. This high-throughput experiment uncovers a novel host response against virus infection, and will be useful for understanding the whole picture of virus pathogenesis.

Samples

We used two major target cell type for RPV infection, a lymphoid cell line (COBL-a cells) and an epithelial cell line (HEK293 cells stably expressing receptor SLAM; 293SLAM cells) [1]. In addition, we established a reverse genetics for RPV-L strain, which is highly virulent against rabbit [2,3], and succeeded in generating recombinant RPV lacking C protein. Cells were infected with each RPV at a multiplicity of infection of 2, and were harvested at 6, 12, 24 and 48 h post infection. Three biological triplicates were performed.

Quality control

No marker genes are established yet. Infection, replication and growth of virus were verified by cytopathic effect of cells (formation of multinuclear giant cells).

References

[1] Measles virus induces cell-type specific changes in gene expression. Sato H1, Honma R, Yoneda M, Miura R, Tsukiyama-Kohara K, Ikeda F, Seki T, Watanabe S, Kai C. Virology. 2008 Jun 5;375(2):321-30. PMID:18374960

[2] Rinderpest virus H protein: role in determining host range in rabbits. Yoneda M, Bandyopadhyay SK, Shiotani M, Fujita K, Nuntaprasert A, Miura R, Baron MD, Barrett T, Kai C. J Gen Virol. 2002 Jun;83(Pt 6):1457-63. PMID:12029161

[3] Rinderpest virus phosphoprotein gene is a major determinant of species-specific pathogenicity. Yoneda M, Miura R, Barrett T, Tsukiyama-Kohara K, Kai C. J Virol. 2004 Jun;78(12):6676-81. PMID:15163758

Beginning of non-public section

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Zenbu configurations and status

• mapping against human genome
  • Rinderpest
  • Rinderpest timecourse - update Oct 26th
• mapping agaisnst RPV L (WT)
  • mappings against RPV (WT)

Expression profiles

• Gene and CAGE cluster expression for the rinderpest series
• https://fantom5-collaboration.gsc.riken.jp/webdav/home/arner/timecourse/time_course_main_paper_freeze_feb2013/qc_release_130226/human_rinderpest/expression_tables/

MARA based network results

• https://fantom5-collaboration.gsc.riken.jp/webdav/home/arner/timecourse/time_course_main_paper_freeze_feb2013/qc_release_130226/human_rinderpest/mara/

Related samples

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References

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Quality control

https://fantom5-collaboration.gsc.riken.jp/webdav/home/arner/timecourse/time_course_main_paper_freeze_feb2013/qc_release_130226/human_rinderpest/

ISMARA analysis results

All samples: http://ismara.unibas.ch/timecourses/rinderpest_all/ismara_report/index.html

For more information, see ISMARA.