OP-RNA-extraction-totalRNA-miRNeasy Mini-v1.0
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Protocol: OP-RNA-extraction-totalRNA-miRNeasy_Mini-v1.0
Protocol name: miRNEasy total RNA extraction protocol
Version: v1.0
Author: Qiagen
Created: 2007/07/01
Description:
Contents:
1. Standard protocol from QIAGEN in English.
2. Notes on using Trizol or Isogen lysates.
3. Notes on adapting RNEasy midi and maxi columns to achieve a miRNEasy midi/maxi prep.
miRNeasy Mini Handbook - English (PDF)
URL: http://www.qiagen.com/literature/render.aspx?id=23770
Notes: Note RNeasy and miRNeasy use the same columns, but the ethanol concentration is adjusted to make sure the small RNAs stay on the column. miRNeasy uses a Trizol/qiazol/Isogen pahse separation first and then cleans up the aqueous phase using the RNeasy columns
