Calcification

Saos-2 osteosarcoma model of Calcification/bone deposition

A human model of bone deposition/calcification using teh Saos-2 osteosarcome cell line treated with ascorbic acid and BGP was provided by Kim Summers and Margaret Davis.

Zenbu configurations and status

• Saos-2 osteosarcoma treated with ascorbic acid and BGP(COMPLETED 18 timepoints in triplicate)

Gene expression profiles

• Known gene expression for the Saos-2 series
• Promoterome v1.0 expression for the Saos-2 series
• Top dynamic TFs in Saos-2 series

Motifs

• Top dynamic motifs in Saos-2 series

MARA based network results

• Saos-2 calcification network based on top 30 dynamic motifs

Related samples

• Osteosarcoma cell lines 143B/TK^(-)neo^(R) and HS-Os-1 from RIKEN BRC - contact Al Forrest

References

• First paper on K562: http://www.ncbi.nlm.nih.gov/pubmed/789258
• First paper on J2E: http://www.pnas.org/content/85/22/8506.full.pdf

Quality control

• staining pictures...