Calcification

Saos-2 osteosarcoma model of Calcification/bone deposition

A human model of bone deposition/calcification using the Saos-2 osteosarcoma cell line treated with ascorbic acid and BGP was provided by Kim Summers and Margaret Davis.

Zenbu configurations and status

• Saos-2 osteosarcoma treated with ascorbic acid and BGP(COMPLETED 18 timepoints in triplicate)

Gene expression profiles

• Known gene expression for the Saos-2 series (RefSeq based promoters)
  • TPM
  • tagcount

• Promoterome v1.0 expression for the Saos-2 series
• Top dynamic TFs in Saos-2 series
• OSC Cluster expression for the Saos-2 series
  • https://fantom5-collaboration.gsc.riken.jp/webdav/home/m.lizio/20111011_other_expression_tables_by_timecourse/human.Saos-2.hCAGE.hg19.tpm.L2clusters.osc.txt.gz

Motifs

• Top dynamic motifs in Saos-2 series

MARA based network results

• Saos-2 calcification network based on top 30 dynamic motifs
  • https://fantom5-collaboration.gsc.riken.jp/webdav/home/m.lizio/20111011_other_expression_tables_by_timecourse/human.Saos-2.hCAGE.hg19.tpm.MARA.txt.gz

Related samples

• Osteosarcoma cell lines 143B/TK^(-)neo^(R) and HS-Os-1 from RIKEN BRC - contact Al Forrest
• Additional untreated timecourse is stored
• Additional timecourse using MG63 is stored

References

• First paper on Saos-2: http://www.ncbi.nlm.nih.gov/pubmed/3040234

Quality control

• Staining pictures...