Retinal pigment mesenchyme transition: Difference between revisions
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[mailto:ogishima@sysmedbio.org, Soichi Ogishima], [mailto:tanaka@bioinfo.tmd.ac.jp, Hiroshi Tanaka], [mailto:miyaguchi@bioinfo.tmd.ac.jp, Ken Miyaguchi], [mailto:eslami@bioinfo.tmd.ac.jp, Afsaneh Eslami] |
[mailto:ogishima@sysmedbio.org, Soichi Ogishima], [mailto:tanaka@bioinfo.tmd.ac.jp, Hiroshi Tanaka], [mailto:miyaguchi@bioinfo.tmd.ac.jp, Ken Miyaguchi], [mailto:eslami@bioinfo.tmd.ac.jp, Afsaneh Eslami] |
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= Introduction<br> = |
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= Samples<br> = |
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<br>ARPE-19 cells were cultured in Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 medium (Sigma-Aldrich) with 10% fetal bovine serum in a CO2 incubator at 37 °C. After 5 days of preculture, cells were treated with human recombinant TGF-β2 and human recombinant TNF-α and continuously incubated in serum free medium at 37 °C to induce EMT. At 21 different time points (0 min, 15 min, 30 min, 45 min, 60 min, 80 min, 100 min, 2 hr, 2.5 hr, 3 hr, 3.5 hr, 4 hr, 5 hr, 6 hr, 7 hr, 8 hr, 12 hr, 16 hr, 24 hr, 42 hr and 60 hr), cells were dissolved in lysis reagent and collected. For the samples of 0 min, cells were treated with PBS without TGF-β2 and TNF-α. Using miRNeasy Mini Kit (QIAGEN), total RNA was extracted from each cell lysate according to a manufacturer’s protocol.<br> |
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= Quality control<br> = |
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= References<br> = |
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[1] Tumor necrosis factor-alpha regulates transforming growth factor-beta-dependent epithelial-mesenchymal transition by promoting hyaluronan-CD44-moesin interaction. Takahachi E et al, J Biol Chem, 2010, 285(6):4060-73. [http://www.ncbi.nlm.nih.gov/pubmed/19965872 PMID:19965872]<br> |
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[2] Epithelial-mesenchymal transitions in development and disease, Thiery JP et al, Cell, 2009, 139(5):871–890. [http://www.ncbi.nlm.nih.gov/pubmed/19945376 PMID:19945376]<br> |
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Revision as of 06:11, 21 July 2014
Editing Retinal pigment mesenchyme transition
Time course ID: human_ARPE-19
Sample provider:
Soichi Ogishima, Hiroshi Tanaka, Ken Miyaguchi, Afsaneh Eslami
Introduction
Samples
ARPE-19 cells were cultured in Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 medium (Sigma-Aldrich) with 10% fetal bovine serum in a CO2 incubator at 37 °C. After 5 days of preculture, cells were treated with human recombinant TGF-β2 and human recombinant TNF-α and continuously incubated in serum free medium at 37 °C to induce EMT. At 21 different time points (0 min, 15 min, 30 min, 45 min, 60 min, 80 min, 100 min, 2 hr, 2.5 hr, 3 hr, 3.5 hr, 4 hr, 5 hr, 6 hr, 7 hr, 8 hr, 12 hr, 16 hr, 24 hr, 42 hr and 60 hr), cells were dissolved in lysis reagent and collected. For the samples of 0 min, cells were treated with PBS without TGF-β2 and TNF-α. Using miRNeasy Mini Kit (QIAGEN), total RNA was extracted from each cell lysate according to a manufacturer’s protocol.
Quality control
References
[1] Tumor necrosis factor-alpha regulates transforming growth factor-beta-dependent epithelial-mesenchymal transition by promoting hyaluronan-CD44-moesin interaction. Takahachi E et al, J Biol Chem, 2010, 285(6):4060-73. PMID:19965872
[2] Epithelial-mesenchymal transitions in development and disease, Thiery JP et al, Cell, 2009, 139(5):871–890. PMID:19945376
...
Zenbu configurations and status
Expression profiles
- Gene and CAGE cluster expression for the retinal epithelium series
- https://fantom5-collaboration.gsc.riken.jp/webdav/home/arner/timecourse/time_course_main_paper_freeze_feb2013/qc_release_130226/human_ARPE-19/expression_tables/
MARA based network results
Related samples
- ...
References
- ...
Quality control
ISMARA analysis results
All samples: http://ismara.unibas.ch/timecourses/retinal_pigment_EMT/ismara_report/index.html
Replicate averaged: http://ismara.unibas.ch/timecourses/ARPE19-avgd/averaged_report/index.html
For more information, see ISMARA.
