Noncoding RNA central page: Difference between revisions

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=ZENBU configuration=
=ZENBU configuration=
This [https://fantom5-collaboration.gsc.riken.jp/zenbu/gLyphs/#config=AaQFgy8gHxzvxJHTnzYiHB;loc=hg19::chr7:41712147..41840341 link] leads to a ZENBU configuration containing tracks with pooled hCAGE, pooled short RNA, and pooled FANTOM5 RNA-seq expression data (edit: 11/9/2001--added CAGE-scan clusters, see F5 mailing list message 01096). Transcript structure is also provided in pooled CAGE-scan and RNA-seq tracks.
This [https://fantom5-collaboration.gsc.riken.jp/zenbu/gLyphs/#config=bFijo7RhLRqlynp6K8LaSB;loc=hg19::chr7:41712147..41840341 link] leads to a ZENBU configuration containing tracks with pooled hCAGE, pooled short RNA, and pooled FANTOM5 RNA-seq expression data (edit: 11/9/2001--added CAGE-scan clusters, see F5 mailing list message 01096). Transcript structure is also provided in pooled CAGE-scan and RNA-seq tracks.


A couple of warnings:
A couple of warnings:

Revision as of 10:52, 8 December 2011

Welcome to the central noncoding RNA page. This page serves as a repository for links to data of potential interest to computational collaborators, processed data hopefully of interest to wet-bench collaborators, and FANTOM5 paper pages centered on ncRNA analysis.

ZENBU configuration

This link leads to a ZENBU configuration containing tracks with pooled hCAGE, pooled short RNA, and pooled FANTOM5 RNA-seq expression data (edit: 11/9/2001--added CAGE-scan clusters, see F5 mailing list message 01096). Transcript structure is also provided in pooled CAGE-scan and RNA-seq tracks.

A couple of warnings:

  1. CAGE-scan/RNA-seq transcripts could crash your browser at loci with a large amount of expression.
  2. there are serious issues with short RNA expression values rooted in improper normalization.

We are aware of both of these issues; for issue #1 we are experimenting with new ways to view transcript structure and for issue #2 we are working on re-mapping and re-normalization of the short RNA data.


November 11th update

The latest "F5 human lncRNAome 05Nov11 ((H_JIA)) (n=20947)" () is now available

  1. As annotation (remember to select "bed_region" to obtain the full trancsript like complete structure)
  2. As a single track in DEX (somewhat more convenient that selecting it as an "annotation")
  3. Under this link, the latest lncRNAome dataset now replaces the previous ("F5_human_lncRNAome_28July2011") one.
  4. In addition the (latest as of November 11th) complete F5 human sample pooled expression ("Helicos all CTSS pooled human update Oct-25-2011 1409 samples") along with the updated F5 human lncRNAome is accessible thru the following link

To circumvent the issue pertaining to CAGE-scan potentially crashing your browser at loci with a large amount of expression, I recommend solely looking at the CAGEscan cluster track displaying the data as transcript like structures (the expression track not being particularly more informative than helicos CAGE data when expression levels are of interest).

FANTOM5 paper pages

  • long non-coding RNA paper centered on chains/networks of lncRNAs and coding RNAs
  • FANTOM5 novel lncRNA search with RNA-seq (likely to be folded into the main paper above)
  • small RNA paper (some of this will be included in lncRNA paper but there is likely enough here to stand on its own as a separate paper)

Data for computational researchers

long noncoding RNA (lncRNA)

small RNA

  • link to raw data (forthcoming, target: Nov. 15)
  • miRNA expression table with normalization factors (forthcoming, target: Nov. 15)

RNA-seq

This also includes coding RNA, of course!

  • link to currently-available raw data, ~18 libraries in twin samples of whole blood, CD19+, CD8+ (forthcoming)
  • Cufflinks-assembled transcripts for the same ~18 libraries (forthcoming, target: Nov. 15)
  • link to list of tissues selected for RNA-seq File:F5 RNAseq library list.xlsx
  • link to ~50 RNA-seq libraries raw data (~end of January)
  • link to Cufflinks-assembled transcripts for the ~50 libraries (~mid-February)
  • additional public data processed for our benefit! (soon, suggestions for good datasets to poach (ha! Alistair word!) are welcome)

CAGEscan

  • links to expression tables

Processed data of potential interest to wet-bench collaborators

As we don't have the initial processing of the data completed, these aren't quite ready yet. But we will update! Suggestions are more than welcome. We plan to make these available as excel/tab-delimited files with library name and ncRNA identifier (lncRNA, miRNA locus name, etc.) to look up in ZENBU.

lncRNA-related

  • tissue-specific lncRNAs of potential interest
  • lncRNAs displaying differential expression during a timecourse

small RNA

  • primary cell-specific novel small RNA expression
  • differential miRNA expression
  • novel miRNAs of interest/tissue specific
  • differential promoter RNA expression
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